کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8356702 | 1542019 | 2018 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CYSTATHIONINE GAMMA-SYNTHASE activity in rice is developmentally regulated and strongly correlated with sulfate
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کلمات کلیدی
SAAO-acetylserineSMMHcysDMSSATGSHOPASAMSO42− - SO42-branched chain amino acids - آمینو اسیدهای زنجیرهای منشعب شدهS-adenosylmethionine - اس-ادنوزیل متیونینSulfur amino acid - اسید آمینه سولفوریکaromatic amino acids - اسید آمینه معطرThreonine synthase - تراتونین سنتازDimethylsulfide - دی متیل سولفیدAnthranilate synthase - سنتاز آنترونیلاتSulfate - سولفاتChorismate mutase - موتاز کوریسماتResponse ratio - نسبت پاسخHomoserine - هوموسرینhomocysteine - هوموسیستئینOAS - واحهGamma-glutamylcysteine synthetase - گاما گلوتامیل سیستئین سنتتازGlutathione - گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
An important goal of rice cultivar development is improvement of protein quality, especially with respect to essential amino acids such as methionine. With the goal of increasing seed methionine content, we generated Oryza sativa ssp. japonica cv. Taipei 309 transgenic lines expressing a feedback-desensitized CYSTATHIONINE GAMMA-SYNTHASE from Arabidopsis thaliana (AtD-CGS) under the control of the maize ubiquitin promoter. Despite persistently elevated cystathionine gamma-synthase (CGS) activity in the AtD-CGS transgenic lines relative to untransformed Taipei, sulfate was the only sulfur-containing compound found to be elevated throughout vegetative development. Accumulation of methionine and other sulfur-containing metabolites was limited to the leaves of young plants. Sulfate concentration was found to strongly and positively correlate with CGS activity across vegetative development, irrespective of whether the activity was provided by the endogenous rice CGS or by a combination of endogenous and AtD-CGS. Conversely, the concentrations of glutathione, valine, and leucine were clearly negatively correlated with CGS activity in the same tissues. We also observed a strong decrease in CGS activity in both untransformed Taipei and the AtD-CGS transgenic lines as the plants approached heading stage. The mechanism for this downregulation is currently unknown and of potential importance for efforts to increase methionine content in rice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 270, May 2018, Pages 234-244
Journal: Plant Science - Volume 270, May 2018, Pages 234-244
نویسندگان
Sarah J. Whitcomb, Huu Cuong Nguyen, Franziska Brückner, Holger Hesse, Rainer Hoefgen,