کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8356741 | 1542020 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Integrating proteomics and enzymatic profiling to decipher seed metabolism affected by temperature in seed dormancy and germination
ترجمه فارسی عنوان
ادغام پروتئومیک و پروفایل آنزیمی برای تشریح سوخت و ساز بذر تحت تاثیر دما در خواب و جوانه زنی بذر
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کلمات کلیدی
درجه حرارت، خوابیدن دانه جوانه زنی، پروتئومیکس، نمایه سازی آنزیمی، متابولیسم،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
چکیده انگلیسی
Temperature is an important environmental factor affecting seed dormancy and germination. The mechanism by which temperature induces germination in dormant seeds is however still unclear. Proteomic study has been performed in dormant sunflower seeds during imbibition at permissive and non-permissive temperatures for germination, 20 and 10â¯Â°C, respectively. Proteome analysis showed an increase of proteins belonging to metabolism and energy from the first hours of imbibition followed by a decrease of proteins involved in protein metabolism and seed storage in germinating compared to non-germinating seeds. Proteomic study was completed by polysome and proteasome activity assessment and enzymatic profiling on several altered proteins involved in metabolism and energy. Results showed that 20â¯Â°C treatment induced the activation of both protein synthesis and degradation processes, the latter being related to proteasome activity during the germination sensu stricto, and to other degradation processes such as proteases during the post-germination. Interestingly, enzymatic profiles showed that TCA cycle and glycolysis were more active in non-germinating seeds in the phase I of the germination sensu stricto. This result suggests the regulation of central metabolism activity in germinating seeds. The control of energy production during imbibition seems to be involved in molecular networks controlling seed dormancy and germination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 269, April 2018, Pages 118-125
Journal: Plant Science - Volume 269, April 2018, Pages 118-125
نویسندگان
Qiong Xia, Maharajah Ponnaiah, Gwendal Cueff, Loïc Rajjou, Duyen Prodhomme, Yves Gibon, Christophe Bailly, Françoise Corbineau, Patrice Meimoun, Hayat El-Maarouf-Bouteau,