کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357813 | 1542055 | 2015 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the heterotrimeric G-protein family and its transmembrane regulator from capsicum (Capsicum annuum L.)
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کلمات کلیدی
AggGPARGSGTPADPHeterotrimeric G-proteins - Heterotrimeric G-پروتئین هاAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPadenosine diphosphate - آدنوزین دی فسفاتBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیBlast - انفجارProtein–protein interaction - تعامل پروتئین-پروتئینFruit development - توسعه میوهGDP - تولید ناخالص ملیRegulator of G-protein signaling - رگولاتور سیگنالینگ G-proteinRegulator of G protein signaling - رگولاتور سیگنالینگ پروتئین GCapsicum annuum - سیب زمینیAGB - شرایطGAP - شکافGuanosine triphosphate - گوانوزین تری فسفاتguanosine diphosphate - گوانوزین دی فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Throughout evolution, organisms have created numerous mechanisms to sense and respond to their environment. One such highly conserved mechanism involves regulation by heterotrimeric G-protein complex comprised of alpha (Gα), beta (Gβ) and gamma (Gγ) subunits. In plants, these proteins play important roles in signal transduction pathways related to growth and development including response to biotic and abiotic stresses and consequently affect yield. In this work, we have identified and characterized the complete heterotrimeric G-protein repertoire in the Capsicum annuum (Capsicum) genome which consists of one Gα, one Gβ and three Gγ genes. We have also identified one RGS gene in the Capsicum genome that acts as a regulator of the G-protein signaling. Biochemical activities of the proteins were confirmed by assessing the GTP-binding and GTPase activity of the recombinant Gα protein and its regulation by the GTPase acceleration activity of the RGS protein. Interaction between different subunits was established using yeast- and plant-based analyses. Gene and protein expression profiles of specific G-protein components revealed interesting spatial and temporal regulation patterns, especially during root development and during fruit development and maturation. This research thus details the characterization of the first heterotrimeric G-protein family from a domesticated, commercially important vegetable crop.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 234, May 2015, Pages 97-109
Journal: Plant Science - Volume 234, May 2015, Pages 97-109
نویسندگان
Rafael A. Romero-Castillo, Swarup Roy Choudhury, Josefina León-Félix, Sona Pandey,