کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8358009 | 1542060 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nitrate inhibits soybean nodulation by regulating expression of CLE genes
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کلمات کلیدی
YMBSDIDAICLECaMVGUSqRT-PCRGFPHAR1ORFLRR-RLKSupernodulationcDNA - cDNAComplementary DNA - DNA تکمیلیβ-glucuronidase - β-گلوکورونیدازNodulation - استقرارdays after inoculation - روز بعد از تلقیحquantitative reverse transcription-PCR - رونویسی معکوس PCRleucine-rich repeat receptor-like kinase - غنی از لوسین تکرار گیرنده مانند کینازopen reading frame - قاب خواندن بازLuria–Bertani - لواریا بارتانیwild-type - نوع وحشیNitrate - نیتراتpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازcauliflower mosaic virus - ویروس موزائیک گل کلمgreen fluorescent protein - پروتئین فلورسنت سبزoptical density - چگالی نوریAON - یکم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nitrogen compounds such as nitrate act as a potential inhibitor for legume nodulation. In this study, we isolated a new CLE gene, GmNIC2, from nitrate-treated roots, which shares high sequence homology with nitrate-induced CLE gene GmNIC1. Similar to GmNIC1, the expression level of GmNIC2 was not significantly altered in roots by rhizobial inoculation and was much higher in young nodules than in roots. In addition, overexpression of GmNIC2 led to similar nodulation inhibition of transgenic hairy roots to that of GmNIC1, which occurred in GmNARK-dependent manner and at the local level. By analyzing GmNARK loss-of-function mutant, SS2-2, it was found that expression levels of GmNIC1 and GmNIC2 in the SS2-2 roots were lower than in the wild type (WT) roots in response to nitrate. In contrast to GmNIC1 and GmNIC2, expressions of GmRIC1 and GmRIC2 genes that are related to the autoregulation of nodulation (AON) were strongly suppressed both of the soybeans during all periods of nitrate treatment and even were not induced by additional inoculation with rhizobia. Taken together, the results of this study suggest that GmNIC2, as an active homologous gene located in chromosome 13, acts locally to suppress nodulation, like GmNIC1, and nitrate inhibition of nodulation is led by fine-tuned regulation of both nitrate-induced CLEs and rhizobia-induced CLEs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 229, December 2014, Pages 1-9
Journal: Plant Science - Volume 229, December 2014, Pages 1-9
نویسندگان
Chae Woo Lim, Young Woo Lee, Sung Chul Lee, Cheol Ho Hwang,