کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8358716 1542075 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of potential miRNAs and their targets in Vriesea carinata (Poales, Bromeliaceae)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Identification of potential miRNAs and their targets in Vriesea carinata (Poales, Bromeliaceae)
چکیده انگلیسی
The miRNAs play important roles in regulation of gene expression at the post-transcriptional level. A small RNA and RNA-seq of libraries were constructed to identify miRNAs in Vriesea carinata, a native bromeliad species from Brazilian Atlantic Rainforest. Illumina technology was used to perform high throughput sequencing and data was analyzed using bioinformatics tools. We obtained 2,191,509 mature miRNAs sequences representing 54 conserved families in plant species. Further analysis allowed the prediction of secondary structures for 19 conserved and 16 novel miRNAs. Potential targets were predicted from pre-miRNAs by sequence homology and validated using RTqPCR approach. This study provides the first identification of miRNAs and their potential targets of a bromeliad species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 210, September 2013, Pages 214-223
نویسندگان
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