Effects of nitrogen source and water availability on stem carbohydrates and cellulosic bioethanol traits of alfalfa plants

► We examine changes in stem carbohydrates and cell wall composition under drought. ► Nitrogen-fixing plants have high photosynthesis and stem carbohydrate content. ► Drought deteriorate stem traits suitable for biofuel production in nitrate-fed plants. ► Nodulated plants have high carbohydrates and cell wall cellulose under drought.