کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8359225 | 1542289 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimized production of insulin variant, a recombinant platelet aggregation inhibitor, by high cell-density fermentation of recombinant Escherichia coli
ترجمه فارسی عنوان
تولید بهینه شده از نوع انسولین، بازدارنده تجمع پلاکت های نوترکیب، توسط تخمیر بالای سلول های اشرشیاکلی نوترکیب
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Optimal conditions for a high cell-density fermentation of Escherichia coli strain harboring a recombinant anti-thrombosis insulin variant (named rAT-INS) were investigated by using fed-batch culture employing pH-stat method. The optimized main medium composition were glycerol 10â¯g/L, yeast extract 30â¯g/L, trypton 10â¯g/L, NaCl 5â¯g/L. The late-stage induction with 0.05â¯mM isopropyl-β-d- thiogalactopyranoside showed the highest productivity after 28â¯h of the fed-batch fermentation. This optimized process yielded about 150â¯mg of purified rAT-INS from 1â¯L of wet cell mass with high-homogeneity. The amino acid compositions and mass data of the purified rAT-INS were in good agreement with those as expected. Purified rAT-INS exhibited potent inhibitory activity of platelet aggregation. The in vivo assay showed that rAT-INS had a higher activity in prolonging the bleeding time in mice than native-insulin. The purified rAT-INS had almost no insulin receptor binding activity. Our study demonstrates the promise for mass production of novel recombinant antiplatelet agents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 152, December 2018, Pages 7-12
Journal: Protein Expression and Purification - Volume 152, December 2018, Pages 7-12
نویسندگان
Jian Jing, Yaoling Chen, Li Sheng, Mi Wu,