کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8360465 1542340 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Affinity purification of recombinant human cytochrome P450s 3A4 and 1A2 using mixed micelle systems
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Affinity purification of recombinant human cytochrome P450s 3A4 and 1A2 using mixed micelle systems
چکیده انگلیسی
Recombinant cytochrome P450 (CYP or P450) enzymes are useful for drug metabolism research and thereby many expression and purification systems have been developed. Here, we provide a method for the purification of human P450s 3A4 and 1A2 expressed in Escherichia coli using mixed micelles containing anionic phospholipids. This method does not require any protein-tagging system for protein isolation and has a further advantage that the purification is concomitantly conducted with reconstitution of the enzymes into a phospholipid environment, which is crucial for the catalytic activity assay of P450 enzyme. This method may also be applied to high-throughput catalytic assays of the enzymes because the purification procedures can be undertaken in a 96-well plate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 101, September 2014, Pages 37-41
نویسندگان
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