کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8360596 1542345 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A semi-automated method for purification of milligram quantities of proteins on the QIAcube
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A semi-automated method for purification of milligram quantities of proteins on the QIAcube
چکیده انگلیسی
A growing number of studies require the purification of multiple proteins simultaneously and the development of simple economical high-throughput purification methods is essential. We have tested the purification of two related proteins in a variety of conditions to benchmark the semi-automated affinity chromatography method for the QIAcube that we have developed. We find that this new QIAcube method can successfully purify milligram quantities of proteins with minimal user involvement and performs as well as methods based on gravity. The method could easily be adapted to other chromatography resins and should prove to be a versatile method for optimizing protein expression or purification conditions for multiple proteins while obtaining sufficient amounts for subsequent biochemical analyses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 96, April 2014, Pages 48-53
نویسندگان
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