کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8360750 | 1542349 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction and characterization of a potent, long-lasting recombinant human serum albumin-interferon α1 fusion protein expressed in Pichia pastoris
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Construction and characterization of a potent, long-lasting recombinant human serum albumin-interferon α1 fusion protein expressed in Pichia pastoris Construction and characterization of a potent, long-lasting recombinant human serum albumin-interferon α1 fusion protein expressed in Pichia pastoris](/preview/png/8360750.png)
چکیده انگلیسی
The main obstacles to using Interferon a1 as an antiviral agent are its low stability and fast clearance. Here, we prepared a long-lasting recombinant human serum albumin-interferon α1 fusion protein. It was expressed in methylotrophic yeast Pichia pastoris with HSA's natural signal peptide and purified by dye affinity chromatography and ion exchange chromatography. The physicochemical, biological, and pharmaceutical characteristics of HSA-IFN α1 were then evaluated in vitro and in vivo. The purity of HSA-IFN α1 was about 95% analyzed by SDS-PAGE. Molecular weight determined by MALDI-TOF mass spectrometry was 86,582. Western blot analysis showed that the expressed HSA-IFN α1 had the antigenicity of HSA. The N-terminal acid amino sequence was identical to predicted sequence. The antivirus activity in vitro evaluated by cytopathic effect assay was (1.63 ± 0.06) Ã 105 IU/mg. After administered in rats, the biological activity of HSA-IFN α1 were enhanced and maintained for 6 days in serum. Overall, these studies demonstrate the feasibility of using albumin-fusion technology to development a long-lasting, antiviral protein HSA-IFN α1 with high antivirus activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 90, Issue 2, August 2013, Pages 124-128
Journal: Protein Expression and Purification - Volume 90, Issue 2, August 2013, Pages 124-128
نویسندگان
Shuo Tian, Qinshan Li, Wenbin Yao, Chen Xu,