l-Cysteine-enhanced renaturation of bioactive soluble tumor necrosis factor ligand family member LIGHT from inclusion bodies in Escherichia coli

► Recombinant sLIGHT is expressed as inclusion bodies in E. coli. ► L-Cysteine highly renaturates sLIGHT from inclusion bodies. ► L-Arginine enhances sLIGHT renaturation by L-cysteine from inclusion bodies. ► Renaturated sLIGHT potently inhibits the growth of human cancer cells.