کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8388599 | 1543932 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation
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موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
علوم زمین و سیاره ای (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation](/preview/png/8388599.png)
چکیده انگلیسی
We established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA including electric field strength and plasmid form were optimized. Following optimization, the diatom cells could be transformed with exogenous gene easily, the maximum transformation frequency obtained was 2.8 Ã 10â 5 cells. The cotransformation of P. tricornutum with a non-selective GUS gene together with the selectable resistance gene has also been achieved using our new method and found to be very efficient (up to 60%). The electroporation procedure described in this article offers a number of advantages, including simplicity, general utility, low-cost and high efficiency. The described method also provides some clue for developing electroporation transformation system in other eukaryotic microalgae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Genomics - Volume 16, August 2014, Pages 63-66
Journal: Marine Genomics - Volume 16, August 2014, Pages 63-66
نویسندگان
Chunye Zhang, Hanhua Hu,