کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8392931 1544052 2017 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trehalose During Two Stress Responses in Acanthamoeba: Differentiation Between Encystation and Pseudocyst Formation
ترجمه فارسی عنوان
ترهالوز در طی پاسخ های دو استرس در آتاناموبا: تمایز بین انباشتگی و سازند پوسیدوسیست
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
The non-reducing disaccharide trehalose can serve as a protectant against a range of environmental stressors, such as heat, cold, or dehydration, in both prokaryotes and eukaryotes, with the exception of vertebrates. Here, we analyzed trehalose metabolism in the facultatively parasitic organism Acanthamoeba castellanii, known to respond to unfavorable external conditions by forming two resistant stages: a cyst, produced in the case of chronic stress, and a pseudocyst, formed in reaction to acute stress. The possible role of trehalose in the resistant stages was investigated using a combination of bioinformatic, molecular biological and biochemical approaches. Genes for enzymes from a widespread trehalose-6-synthase-trehalose-6-phosphate phosphatase (TPS-TPP) pathway and a prokaryotic trehalose synthase (TreS) pathway were identified. The expression patterns of the genes during encystation and pseudocyst formation were analyzed and correlated with the time course of cellular trehalose content determined mass spectrometrically. The data clearly demonstrate fundamental differences between encystation and pseudocyst formation at the level of cellular metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protist - Volume 168, Issue 6, December 2017, Pages 649-662
نویسندگان
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