کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8405829 | 1544897 | 2018 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Solid-state cultivation of recombinant Aspergillus nidulans to co-produce xylanase, arabinofuranosidase, and xylooligosaccharides from soybean fibre
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Solid-state cultivation of recombinant Aspergillus nidulans to co-produce xylanase, arabinofuranosidase, and xylooligosaccharides from soybean fibre Solid-state cultivation of recombinant Aspergillus nidulans to co-produce xylanase, arabinofuranosidase, and xylooligosaccharides from soybean fibre](/preview/png/8405829.png)
چکیده انگلیسی
Solid-state cultivations of genetically modified strains of Aspergillus nidulans A773, using soybean fibre as substrate, were carried out to produce xylanase and arabinofuranosidase, and these enzymes were subsequently used to obtain xylooligosaccharides using the same agro-residue. First, the best fungi cultivation conditions (moisture content, pH, temperature and addition of maltose) were optimized one-by-one for obtain the crude enzyme extracts. Subsequently, the application of xylanase on soybean fibre to obtain xylooligosaccharides was optimized by central composite design, defining best enzyme concentration and reaction temperature. The best condition obtained (50â¯Â°C and 117 Uâ¯gâ1 of soybean fibre) was used to evaluate the co-production of xylooligosaccharides by the addition of different concentrations of arabinofuranosidase. The highest yield of xylooligosaccharides obtained was 28% (mass fraction of xylan), showing final concentrations (in mgâ¯gâ1 arabino-xylan) of 138.36 xylobiose (X2), 96.96 xylotriose (X3), and 53.04 xylotetraose (X4), in 9â¯h enzymatic reactions. The conversion of arabino-xylans into different xylooligosaccharides suggests the potential to use recombinant A. nidulans A773 enzymes to obtain prebiotics using a sugar-rich, low-cost soybean residue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 78-85
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 78-85
نویسندگان
Gabriela Feix Pereira, Daniela de Bastiani, Sabrina Gabardo, Fabio Squina, Marco Antônio Záchia Ayub,