کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8408755 | 1545077 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10-12Â days. Using this method, 90%-99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%-60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Crop Journal - Volume 6, Issue 2, April 2018, Pages 162-171
Journal: The Crop Journal - Volume 6, Issue 2, April 2018, Pages 162-171
نویسندگان
Li Chen, Yupeng Cai, Xiujie Liu, Chen Guo, Shi Sun, Cunxiang Wu, Bingjun Jiang, Tianfu Han, Wensheng Hou,