کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8415573 | 1545523 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of high hydrostatic pressure on Rhizopus chinensis lipase: II. Intermediate states during unfolding
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
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![عکس صفحه اول مقاله: Effects of high hydrostatic pressure on Rhizopus chinensis lipase: II. Intermediate states during unfolding Effects of high hydrostatic pressure on Rhizopus chinensis lipase: II. Intermediate states during unfolding](/preview/png/8415573.png)
چکیده انگلیسی
High hydrostatic pressure (HHP) is currently considered a well-established technology for processing food and biological materials and there is an interest in investigating the changes in the structural and functional properties of these materials after high pressure treatment. Therefore, the changes in the structure of Rhizopus chinensis lipase (RCL) after high hydrostatic pressure treatment were investigated. Far-UV circular dichroism (CD) spectra showed that the secondary structure of RCL is maintained at pressures below 400Â MPa and becomes gradually disordered after higher pressures are applied. Near-UV CD spectra showed that the RCL begins to lose its tertiary structure at pressure over 400Â MPa. Fluorescence quenching and the binding of 1-anilinonaphtha-lene-8-sulfonate confirmed that a partially unfolded intermediate, with loosely compacted conformation and hydrophobic regions, is formed at a pressure of 600Â MPa. These results also suggest that RCL maintains a native-like state at pressures below 400Â MPa. Above 500Â MPa RCL molecules showed characteristics of being in a molten globule state. Dynamic light scattering (DLS) and atomic force microscopy (AFM) measurements indicated that RCL molecules at these pressures are aggregating. The addition of (NH4)2SO4 to the protein solution could prevent the aggregation, and at 600Â MPa the molecule had a hydrodynamic radius approximately 8% larger than that observed for the control sample, which was regarded as being in the molten globule state. The observations suggest that at increasing pressures, the unfolding mechanism of RCL follows well-defined steps from a native state via a native-like structure ending in molten globular state or molecular aggregation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Innovative Food Science & Emerging Technologies - Volume 45, February 2018, Pages 152-160
Journal: Innovative Food Science & Emerging Technologies - Volume 45, February 2018, Pages 152-160
نویسندگان
Gang Chen, Ming Miao, Bo Jiang, Jian Jin, Osvaldo H. Campanella, Biao Feng,