کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8416362 | 1545571 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genetic variation in wild and hatchery populations of giant freshwater prawn (Macrobrachium rosenbergii) revealed by randomly amplified polymorphic DNA markers
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کلمات کلیدی
tris-acetate-EDTAobserved number of allelesPLSPost larvaeMgCl2SIJμMRAPDμLRandomly amplified polymorphic DNA - DNA پلیمورفیک به طور تصادفی تقویت شده استDNA - DNA یا اسید دزوکسی ریبونوکلئیکdNTPS - DNTPSdeoxyribonucleic acid - اسید deoxyribonucleicdeoxyribonucleotide triphosphates - تری فسفاتهای deoxyribonucleotideeffective number of alleles - تعداد موثر آللGene diversity - تنوع ژنتیکیTAE - توGene flow - جریان ژنbase pair - جفت پایهPopulation structure - ساختار جمعیتHour - ساعتCelsius - سانتیگرادGenetic distance - فاصله ژنتیکیMacrobrachium rosenbergii - ماکروبراکیوم روزنبرژیای یا شاه میگوی رودخانهایMicroliter - میکرولیترnanogram - نانوگرمRAPD markers - نشانگرهای RAPDVOLT - ولتاژPolymorphism - پلی مورفیسمMagnesium chloride - کلرید منیزیمkilobase - کیلوبایز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sustainable improvement and conservation of any genetic resource depend on the assessment of its intra- and inter-population genetic variation. In order to estimate genetic variation in both wild and hatchery populations of Macrobrachium rosenbergii, randomly amplified polymorphic DNA (RAPD) analysis was performed. Analyses of 51 polymorphic loci amplified from genomic DNA by three decamer random primers revealed different degrees of genetic variation in two wild (Bhairab and Rupsha rivers) and hatchery-derived gher (Gher-1 and Gher-2) populations. The proportion of polymorphic loci was found to be higher in wild populations (0.90 and 0.65 for the Bhairab and Rupsha populations, respectively) than the hatchery-derived gher populations (0.29 and 0.16 for Gher-1 and Gher-2, respectively). Likewise, the river populations contained higher levels of gene diversity (0.221 and 0.179 for Bhairab and Rupsha populations, respectively) than the gher populations (0.114 and 0.045 for Gher-1 and Gher-2, respectively). These results suggest reduction of genetic variation and heterozygosity in the hatchery-derived gher populations. Inter-population similarity indices and pairwise genetic distance values showed that variation between the wild or between the gher populations were lower than those between the wild and hatchery populations. On average, 14 loci exhibited significant deviation from homogeneity in wild vs hatchery population pairs, whereas 2 and 3 loci showed heterogeneity in Gher-1 vs Gher-2 and Bhairab vs Rupsha population pairs, respectively. A genetic distance-based UPGMA dendrogram segregated river populations from the gher populations. Our study, therefore, revealed substantial levels of genetic variation between wild and hatchery populations of M. rosenbergii.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetic Engineering and Biotechnology - Volume 15, Issue 1, June 2017, Pages 23-30
Journal: Journal of Genetic Engineering and Biotechnology - Volume 15, Issue 1, June 2017, Pages 23-30
نویسندگان
Babul Bala, Mithun Mallik, Saidin Saclain, Md. Shahidul Islam,