کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8416574 | 1545573 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Somatic embryogenesis and plant regeneration of Capsicum baccatum L.
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کلمات کلیدی
thidiazuronPicloramGA3TDZNaAIAA2,4-D - 2،4-D2,4-dichlorophenoxyacetic acid - 2،4-dichlorophenoxyacetic اسید2,4,5-T - 2،4،5-TMurashige and Skoog (1962) medium - Murashige و Skoog (1962) متوسط استindole-3-acetic acid - اسید Indole-3-استیکα-naphthalene acetic acid - اسید استیک اسید α-نفتالینLeaf - برگsomatic embryos - جنین های سمیgibberellic acid - جیبرلیک اسیدPepper - فلفلCotyledon - کلوچهkinetin - کینتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Somatic embryogenesis and plant regeneration of Capsicum baccatum L. Somatic embryogenesis and plant regeneration of Capsicum baccatum L.](/preview/png/8416574.png)
چکیده انگلیسی
A plant regeneration protocol via somatic embryogenesis was achieved in cotyledon and leaf explants of Capsicum baccatum, when cultured on MS medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D, 0.5-5.0 mg lâ1) in combination with Kinetin (Kn, 0.5 mg lâ1) and 3% sucrose. Various stages were observed during the development of somatic embryos, including globular, heart, and torpedo-stages. Torpedo stage embryos were separated from the explants and subcultured on medium supplemented with various concentrations of different plant growth regulators for maturation. Maximum percentage (55%) of somatic embryo germination and plantlet formation was found at 1.0 mg lâ1 BA. Finally, about 68% of plantlets were successfully established under field conditions. The regenerated plants were morphologically normal, fertile and able to set viable seeds.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 55-60
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 55-60
نویسندگان
Peddaboina Venkataiah, Pittampalli Bhanuprakash, Sadhu Suman Kalyan, Karampuri Subhash,