کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8416683 | 1545649 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Maximizing the immunological output of the cervicovaginal explant model
ترجمه فارسی عنوان
حداکثر سازی خروجی ایمونولوژیک از مدل اشکالات سرویکوواژینال
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کلمات کلیدی
TLRIFN- γMucosal-associated invariant T cellsiNKTSTIAGCHSV-2TRMLPSα-galactosylceramide - α-گالاکتوسیلسرامیدMucosal immunology - ایمونولوژی مخاطیinterferon-γ - اینترفرون-γToll-like receptor - تیالآرSexually transmitted infections - عفونت های منتقله از راه جنسیlipopolysaccharide - لیپوپلی ساکاریدMAIT - مهمانTissue culture - کشت بافت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
In the field of sexually transmitted infections (STI), the cervicovaginal explant (CVEx) model, not only provides the opportunity to study the different immunological arms present in these tissues under steady state conditions, but also their response against ex vivo infection with relevant pathogens. The methodology associated to the establishment of the HIV infection model in the cervicovaginal tissue was described in detail by Grivel et al. earlier (Grivel and Margolis, 2009). With this model as a foundation, we illustrate different approaches to obtain a large number of immunological readouts from a single piece of tissue, thus maximizing the immunological output obtained. Additionally, we discuss several ideas to study some of the immunological subsets present in this mucosal tissue by enriching them with the addition of distinct chemokines or specifically inducing their activation. Importantly, most of the methodology and concepts proposed here can be applied to study the immune subsets resident in other tissues. In the field of mucosal immunology, the possibility of studying resident immune subsets from tissue explants offers a great opportunity to understand the real players against invading pathogens and localized pathologies. Furthermore, this model allows for addressing the therapeutic benefit of modulating the activity of certain molecules and immune subsets against invading pathogens, which may infer their contribution to pathogen control and direct novel therapeutic interventions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 460, September 2018, Pages 26-35
Journal: Journal of Immunological Methods - Volume 460, September 2018, Pages 26-35
نویسندگان
Jon Cantero, Meritxell Genescà ,