کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8418023 | 1545710 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of sandwich ELISAs for the detection of aromatic diisocyanate adducts
ترجمه فارسی عنوان
توسعه الایزا ساندویچ برای تشخیص ادوات دی ایزوسیانات آروماتیک
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کلمات کلیدی
mAbHSATDIHDIMDIhexamethylene diisocyanateOccupational asthma - آسم شغلیMonoclonal antibody - آنتی بادی مونوکلونالenzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استELISA - تست الیزاtoluene diisocyanate - تولئین دی ایزوسیاناتDiisocyanate - دیسیوسیاناتMethylene diphenyl diisocyanate - متیلن دیفنیل دی ایزوسیاناتImmunoassay - واکنش های ایمونواسی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
Diisocyanates (dNCOs) are highly reactive low molecular weight chemicals commonly used in the manufacturing industry. Occupational exposures to dNCOs have been shown to elicit allergic sensitization and occupational asthma. Among the most commonly used dNCOs in industry are the aromatic dNCOs, toluene diisocyanate (TDI) and methylene diphenyl diisocyanate (MDI). This study aimed to develop enzyme linked immunosorbent assays (ELISA) utilizing aromatic dNCO-specific monoclonal antibodies (mAbs) for the detection of aromatic dNCO adducts. Two sandwich ELISAs were developed. The first sandwich ELISA utilized mAb 60G2 along with an anti-human serum albumin (HSA) polyclonal antibody. This assay detected MDI-, 2,4- and 2,6-TDI-HSA adducts with limits of detection (LOD) of 2.67, <Â 0.10, and 1.70Â ng/mL, respectively. When spiked into human serum, the LOD of this ELISA increased to 34.37, 7.64 and 24.06Â ng/mL, respectively. The second ELISA utilized mAbs 62G5 and 60G2 for capture and detection. This assay was capable of detecting 2,4- and 2,6-TDI-HSA adducts with LODs of <Â 4.90 and 26.92Â ng/mL, respectively, and when spiked in human serum, <Â 4.90 and 95.93Â ng/mL, respectively. This 62G5-60G2 sandwich assay was also able to detect dNCO adducted transferrin, hemoglobin, keratin and actin, but with less sensitivity than dNCO-HSA. The results of this study demonstrate potential application of these ELISAs in the identification and characterization of aromatic dNCO adducts as well as in biomonitoring occupational and environmental dNCO exposures.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 397, Issues 1â2, 29 November 2013, Pages 66-70
Journal: Journal of Immunological Methods - Volume 397, Issues 1â2, 29 November 2013, Pages 66-70
نویسندگان
Angela R. Lemons, Toni A. Bledsoe, Paul D. Siegel, Donald H. Beezhold, Brett J. Green,