کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8418583 | 1545725 | 2012 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prediction of tolerance in children with IgE mediated cow's milk allergy by microarray profiling and chemometric approach
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CMAHRPCMPSPTcow's milk allergy - آلرژی شیر گاوimmunoglobulin - ایمونوگلوبولینELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاSkin prick test - تست خراش پوستیTolerance - تلرانس Food hypersensitivity - حساسیت غذاییHorseradish peroxidase - پراکسیداز هوررادیشCow's milk protein - پروتئین شیر گاوMultivariate - چند متغیرهChemometrics - کمومتریکس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Prediction of tolerance in children with IgE mediated cow's milk allergy by microarray profiling and chemometric approach Prediction of tolerance in children with IgE mediated cow's milk allergy by microarray profiling and chemometric approach](/preview/png/8418583.png)
چکیده انگلیسی
The sera of a retrospective cohort (n = 41) composed of children with well characterized cow's milk allergy collected from multiple visits were analyzed using a protein microarray system measuring four classes of immunoglobulins. The frequency of the visits, age and gender distribution reflected real situation faced by the clinicians at a pediatric reference center for food allergy in São Paulo, Brazil. The profiling array results have shown that total IgG and IgA share similar specificity whilst IgM and in particular IgE are distantly related. The correlation of specificity of IgE and IgA is variable amongst the patients and this relationship cannot be used to predict atopy or the onset of tolerance to milk. The array profiling technique has corroborated the clinical selection criteria for this cohort albeit it clearly suggested that 4 out of the 41 patients might have allergies other than milk origin. There was also a good correlation between the array data and ImmunoCAP results, casein in particular. By using qualitative and quantitative multivariate analysis routines it was possible to produce validated statistical models to predict with reasonable accuracy the onset of tolerance to milk proteins. If expanded to larger study groups, the array profiling in combination with the multivariate techniques show potential to improve the prognostic of milk allergic patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 382, Issues 1â2, 31 August 2012, Pages 48-57
Journal: Journal of Immunological Methods - Volume 382, Issues 1â2, 31 August 2012, Pages 48-57
نویسندگان
F. Wulfert, G. Sanyasi, L. Tongen, L.A. Watanabe, X. Wang, N.K. Renault, F.H. Falcone, C.M.A. Jacob, M.J.C. Alcocer,