In vitro and in vivo transfection of primary phagocytes via microbubble-mediated intraphagosomal sonoporation

► A novel method for the transformation of macrophages with DNA was developed. ► IgG-opsonized lipid-based microbubbles bearing plasmid DNA were synthesized. ► Phagocytosed microbubbles facilitate sonoporation of the phagosome by ultrasound. ► Modest transfection efficiency but well tolerated by cells. ► Method can be used in vitro and in vivo.