کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
842 | 67 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of ProNectin F derivatives on cell attachment and proliferation Effect of ProNectin F derivatives on cell attachment and proliferation](/preview/png/842.png)
ProNectin F (PnF) was chemically modified by introducing some functional groups to prepare various derivatives of primary amino (PnF-N1), tertiary amino (PnF-N3), quaternary ammonium (PnF-N4), carboxyl (PnF-COOH) and sulfonyl groups (PnF-SO3H). When C3H10T1/2 cells were cultured on non-treated dishes coated with the derivatives, the number of mesenchymal cells attached to the culture dishes increased for the coating with PnF-COOH and PnF-SO3H, even at their low adsorption amount. The cytotoxicity was high for the coating of PnF-N1 and PnF-N4 compared with that of the PnF-N3, PnF-COOH and PnF-SO3H. The treatment with integrin α5 and αV antibodies suppressed the cell attachment to the dishes coated with PnF-COOH and PnF-SO3H. The phosphorylation of extracellular signal-regulated kinase (ERK) was upregulated for cells attached to the dishes coated with PnF-COOH and PnF-SO3H, indicating their enhanced proliferation. It is concluded that the chemical derivatization of PnF enhanced the ability of cell attachment and proliferation.
Journal: Acta Biomaterialia - Volume 9, Issue 2, February 2013, Pages 5194–5200