کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8420727 | 1545913 | 2016 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Total combining power: Technique for the evaluation of the quality control process of clostridiosis vaccines
ترجمه فارسی عنوان
مجموع قدرت ترکیب: تکنیک برای ارزیابی فرایند کنترل کیفیت واکسن کلسترادیوز
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
An efficient technique for evaluation of the quality control of vaccines against clostridiosis is described in this study. This technique is capable of quantifying the toxoid of the bacterium Clostridium perfringens Type D, which is commonly found within these vaccines. The described method is performed in vivo to quantify the toxoid, replacing the current predominant approaches that use the titration of toxins before the inactivation process. This method is based on the partial neutralization of a determined dose of antitoxin by testing different doses of the toxoid. In order to guarantee its reliability, it is essential for the technique to be validated. Thus, the technique was tested using the following validation parameters: specificity and selectivity, detection limit, linear correlation, precision and robustness, in agreement with the requirements of regulatory agencies and international committees from around the world. The method was found to be specific, selective, robust, precise, and linear inside a specific concentration range. Therefore, it could be applied to the quality control of clostridiosis vaccines with satisfactory results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 130, November 2016, Pages 164-168
Journal: Journal of Microbiological Methods - Volume 130, November 2016, Pages 164-168
نویسندگان
Igor Viana Brandi, Eliane Macedo Sobrinho Santos, Bruna Mara Aparecida de Carvalho, Carla Adriana Ferreira Durães, Paula Karoline Soares Farias, Roger Shoji Sari, Alex Sander Rodrigues Cangussu, Adalberto Junior,