کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8421574 | 1545928 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MALDI-TOF mass spectrometry fingerprinting: A diagnostic tool to differentiate dematiaceous fungi Stachybotrys chartarum and Stachybotrys chlorohalonata
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کلمات کلیدی
MEAPMKTri5StachybotrysIBTBTSATCCDSMZDPIBSA - BSAbovine serum albumin - آلبومین سرم گاوCHCA - آنها می خواهندFingerprint - اثر انگشتmatrix-assisted laser desorption/ionization time-of-flight mass spectrometry - اسپکترومتر جرمی زمان یخ زدن لیزر جذب / یونیزاسیون ماتریسα-cyano-4-hydroxycinnamic acid - اسید α-سینو-4-هیدروکسی سدیمMaximum likelihood analysis - حداکثر تحلیل احتمالMALDI-TOF mass spectrometry - طیف سنجی جرمی MALDI-TOFmalt extract agar - عصاره مالت آگارFungi - قارچMALDI-TOF MS - مالدی توف MSAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Stachybotrys chartarum and Stachybotrys chlorohalonata are two closely related species. Unambiguous identification of these two species is a challenging task if relying solely on morphological criteria and therefore smarter and less labor-intensive approaches are needed. Here we show that even such closely related species of fungi as S. chartarum and S. chlorohalonata are unequivocally discriminated by their highly reproducible MALDI-TOF-MS fingerprints (matrix assisted laser desorption/ionization time-of-flight mass spectrometry fingerprints). We examined 19 Stachybotrys and one Aspergillus isolate by MALDI-TOF-MS. All but one isolate produced melanin containing conidia on malt extract agar. Mass spectra were obtained in good quality from the analysis of hyaline and darkly pigmented conidia by circumventing the property of melanin which causes signal suppression. MALDI-TOF fingerprint analysis clearly discriminated not only the two morphologically similar species S. chartarum and S. chlorohalonata from each other but separated them precisely from Stachybotrys bisbyi and Aspergillus versicolor isolates. Furthermore, even S. chartarum chemotypes A and S could be differentiated into two distinct groups by their MALDI-TOF fingerprints. The chemotypes of S. chartarum isolates were identified by trichodiene synthase 5 (tri5) sequences prior to mass spectra analysis. Additionally, species identities of all isolates were verified by their 18S rRNA and tri5 gene sequences.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 115, August 2015, Pages 83-88
Journal: Journal of Microbiological Methods - Volume 115, August 2015, Pages 83-88
نویسندگان
Maike Gruenwald, Andreas Rabenstein, Markko Remesch, Jan Kuever,