کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456500 | 1548603 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ionizing radiation-induced gene expression changes in TP53 proficient and deficient glioblastoma cell lines
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کلمات کلیدی
relative expression software toolDNA damage responsesqPCRHRRFDRDSBsSAMGBMNHEJSSBs - SSBchromatin immunoprecipitation - ایمن سازی کروماتینReST - باقی ماندهionizing radiation - تابش یوننده یا پرتوهای یونیزانSignificance analysis of microarray - تجزیه و تحلیل اهمیت میکروارگانیسمDNA repair - ترمیم DNAHomologous recombination repair - تعمیر مجدد هومولوگmutated - جهش یافتهDNA microarrays - ریزش مو DNAsingle-strand breaks - شکاف تک رشته ایdouble-strand breaks - شکست دو ردیفnon-homologous end joining - عدم پیوستن انتهای غیر همولوگfalse discovery rate - میزان کشف کاذبwild type - نوع وحشیquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیPET - پتCHiP - چیپTP53 gene - ژن TP53Glioblastoma - گلیوبلاستوما
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The genetic heterogeneity presented by different cell lines derived from glioblastoma (GBM) seems to influence their responses to antitumoral agents. Although GBM tumors present several genomic alterations, it has been assumed that TP53, frequently mutated in GBM, may to some extent be responsible for differences in cellular responses to antitumor agents, but this is not clear yet. To directly determine the impact of TP53 on GBM response to ionizing radiation, we compared the transcription profiles of four GBM cell lines (two with wild-type (WT) TP53 and two with mutant (MT) TP53) after 8Â Gy of gamma-rays. Transcript profiles of cells analyzed 30Â min and 6Â h after irradiation showed that WT TP53 cells presented a higher number of modulated genes than MT TP53 cells. Our findings also indicate that there are several pathways (apoptosis, DNA repair/stress response, cytoskeleton organization and macromolecule metabolic process) in radiation responses of GBM cell lines that were modulated only in WT TP53 cells (30Â min and 6Â h). Interestingly, the majority of differentially expressed genes did not present the TP53 binding site, suggesting secondary effects of TP53 on transcription. We conclude that radiation-induced changes in transcription profiles of irradiated GBM cell lines mainly depend on the functional status of TP53.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 756, Issues 1â2, 30 August 2013, Pages 46-55
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 756, Issues 1â2, 30 August 2013, Pages 46-55
نویسندگان
P.R.D.V. Godoy, S.S. Mello, D.A.R. Magalhães, F.S. Donaires, P. Nicolucci, E.A. Donadi, G.A. Passos, E.T. Sakamoto-Hojo,