کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456660 | 1548617 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A comparison of DNA damage induced by aflatoxin B1 in hepatocyte-like cells, their progenitor mesenchymal stem cells and CD34+ cells isolated from umbilical cord blood
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کلمات کلیدی
NMOSCFLMPCYP3A4TPOHGFIC50OSMCyPUCBDEXOncostatin-MhBMSCsDMEMMNCsFBSAFB1 - AFB 1BSA - BSADMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoHepatocytes - hepatocytesMTT - MTTAflatoxin B1 - آفلاتوکسین B1bovine serum albumin - آلبومین سرم گاوHES - او هستThrombopoietin - ترومبوپوتیینDifferentiation - تفکیکUmbilical cord blood - خون بند نافDexamethasone - دگزامتازونDimethylsulfoxide - دیمتیل سولفواکسیدComet assay - روش کامت یا روش سنجش ستاره دنبالهدار Na2EDTA - سدیم 2 EDTAfetal bovine serum - سرم جنین گاوStem cell - سلول بنیادیHuman bone marrow mesenchymal stem cells - سلول های بنیادی مزانشیمی مغز استخوان انسانMononuclear cells - سلولهای تک هسته ایCytotoxicity - سمیت سلولیCytochrome P450 - سیتوکروم پی۴۵۰Hepatocyte growth factor - عامل رشد هپاتوسیتinhibitory concentration - غلظت مهاریStem Cell Factor - فاکتور سلول بنیادیNormal melting point - نقطه ذوب طبیعیlow melting point - نقطه ذوب پایینArbitrary Unit - واحد خودآموز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study compared the sensitivity of differentiated hepatocyte-like cells, their progenitor mesenchymal stem cells (MSCs) and CD34+ stem cells to DNA damage and toxicity induced by aflatoxin B1 (AFB1). The hepatocyte-like cells and their progenitor cells (isolated from umbilical cord blood (UCB)) were each treated with AFB1 on day 15 of differentiation. Cell toxicity and genotoxicity effects were assessed using MTT and alkaline comet assays. AFB1 treatment resulted in a dose- and time-dependent inhibition of cell growth. The IC50 values of AFB1 for hepatocytes differentiated from CD34+ and MSCs were within the same range (44.7-46.8 μM). The IC50 calculated for non-differentiated MSCs and CD34+ cells was slightly lower (42.0-43.4 μM) than that calculated for their differentiated counterparts. However, the extent of DNA damage was different in differentiated and non-differentiated cells. The percentages of DNA (% DNA) in comet tails measured in hepatocytes differentiated from MSCs exposed to AFB1 (0, 2.5, 10 and 20 μM) for 24 h were â¼15, 55, 65 and 70%, respectively. In comparison, hepatocytes from CD34+ cells were more resistant to AFB1-induced DNA damage. Hepatocyte-MSCs were most sensitive to DNA damage, followed by UCB-CD34+ cells, then UCB-MSCs and finally hepatocyte-CD34+ cells. These results clearly showed that stem cells from different sources have different sensitivities to DNA damaging agents. These differences can be assigned to the expression levels of cytochrome P450 (CYP) particularly CYP3A4 in non-differentiated and differentiated cells. These data are useful in better understanding the susceptibility/resistance of stem cells in the process of differentiation to environmental toxicants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 719, Issues 1â2, 3 February 2011, Pages 14-20
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 719, Issues 1â2, 3 February 2011, Pages 14-20
نویسندگان
Masoumeh Ghaderi, Abdolamir Allameh, Masoud Soleimani, Hossein Rastegar, Hamid Reza Ahmadi-Ashtiani,