کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456822 | 1548777 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
COX-2 Forms Regulatory Loop with YAP to Promote Proliferation and Tumorigenesis of Hepatocellular Carcinoma Cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
GPCRLats1/2GαsTranscriptional coactivator with PDZ-binding motifCyr61G-protein–coupled receptorTAZAREGEP2PGE2CTGFCOXCelshRNAGAPDH3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide - 3- (4،5-dimethyl-2-thiazolyl) -2،5-difenyl-2-H-tetrazolium bromideHCC - HCCMTT - MTTshort hairpin RNA - RNA موی سر کوتاهamphiregulin - آمفیرگولینcyclooxygenase - آنزیم سیکلواکسیژنازYAP - ایجادchromatin immunoprecipitation - ایمن سازی کروماتینembryonic stem - ساقه جنینCelecoxib - سلکوکسیبConnective tissue growth factor - فاکتور رشد بافت همبندTead - می دانیدneurofibromin 2 - نوروفیبرومین 2wild-type - نوع وحشیHepatoblastoma - هپاتوبلاستوماVerteporfin - ورتپورفینyes-associated protein - پروتئین مرتبط با بلهProstaglandin E2 - پروستاگلاندین E2CHiP - چیپHepatocellular carcinoma - کارسینوم هپاتوسلولار(کارسینوم سلولهای استخوانی)glyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
COX-2 and YAP are shown to be highly associated with hepatocellular carcinoma (HCC) and frequently upregulated during tumor formation. However, despite their importance, whether there is a mutual interaction between COX-2 and YAP and how they regulate each other are not clear. In this paper, we showed that COX-2 overexpression in HCC cell lines resulted in increased levels of YAP mRNA, protein, and its target genes. COX-2 promoted proliferation of HCC cell lines, and knockdown of YAP antagonized this effect. In addition, our results indicated that EP2 and Wnt/β-Catenin mediate the transcriptional induction of YAP by COX-2. On the other hand, YAP increased COX-2 expression at the level of transcription requiring intact TEAD binding sites in the COX-2 promoter. Collectively, these findings indicated that COX-2 is not only a stimulus of YAP but also a target of Hippo-YAP pathway, thus forming a positive feedback circuit, COX-2-PGE2-EP2-Gαs-β-catenin-YAP-COX-2. In a further study, we showed that inhibition of YAP and COX-2 acted synergistically and more efficiently reduced the growth of HCC cells and tumor formation than either of them alone, suggesting that dual governing of YAP and COX-2 may lead to the discovery of promising therapeutic strategies for HCC patients via blocking this positive feedback loop.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neoplasia - Volume 20, Issue 4, April 2018, Pages 324-334
Journal: Neoplasia - Volume 20, Issue 4, April 2018, Pages 324-334
نویسندگان
Guanglin Xu, Ying Wang, Weijie Li, Yuanyuan Cao, Jinling Xu, Ziwei Hu, Yaping Hao, Li Hu, Yawen Sun,