کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8463595 | 1549375 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MIF promotes a differential Th1/Th2/Th17 inflammatory response in human primary cell cultures: Predominance of Th17 cytokine profile in PBMC from healthy subjects and increase of IL-6 and TNF-α in PBMC from active SLE patients
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کلمات کلیدی
IL-17LPSIL-6ISO-1PBMCPSApsoriatic arthritis - آرتریت پسوریاتیکRheumatoid arthritis - آرتریتروماتوئیدHealthy subjects - افراد سالمstandard deviation - انحراف معیارinterferon - اینترفرونIFN - اینترفرون هاinterleukin-17 - اینترلوکین 17interleukin-6 - اینترلوکین ۶tumor necrosis factor alpha - تومور نکروز عامل آلفاperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیMIF - شهرMacrophage migration inhibitory factor - عامل مهارکننده مهاجرت ماکروفاژTNF-α - فاکتور نکروز توموری آلفاSystemic lupus erythematosus - لوپوس اریتماتوی سیستمیکSLE - لوپوس منتشر یا لوپوس اریتماتوس سیستمیکlipopolysaccharide - لیپوپلی ساکاریدSLICC - پروازSystemic Lupus International Collaborating Clinics - کلینیک های همکار همکار سیستمیک لوپوس بین المللی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Macrophage migration Inhibitory Factor (MIF) is a cytokine associated with the pathogenesis of autoimmune inflammatory diseases. There is evidence that MIF functions in a positive-feedback-loop with proinflammatory cytokines and could perpetuate the inflammatory process in Systemic Lupus Erythematosus (SLE).The aim of this study was to assess the effect of recombinant-human-MIF (rhMIF) on the expression of Th1, Th2 and Th17 cytokines in Peripheral Blood Mononuclear Cells (PBMC) from Healthy Subjects (HS) and SLE patients. The PBMC were isolated from SLE patients classified according to the 1997 SLE ACR criteria and HS donors; all subjects included were women from an unrelated Mexican-Mestizo population. The PBMC isolated were stimulated with rhMIF, LPS and ISO-1 in different combinations; Th1, Th2 and Th17cytokine profiles levels were determined by MAGPIX Bio-plex assay in supernatants from cell cultures. We observed in supernatants of PBMCs from HS treated with rhMIF a predominance of Th17 cytokine profile with an increase of IL-17A, IL-17F and IL-21 versus PBMCs from SLE patients, which showed an inflammatory profile represented by increase of IL-6 cytokine. According to SLE remission/activity presented at enrollment in the study (Mex-SLEDAI index), the PBMC from active SLE patients showed higher levels of TNF-α and IL-6 versus PBMC from remission SLE patients. In conclusion, our results suggest that MIF can induce a differential inflammatory response in physiological and pathological conditions with a predominance of a Th17 cytokine profile in PBMC from HS and an increase in TNF-α and IL-6 expression in PBMC from active SLE patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 324, February 2018, Pages 42-49
Journal: Cellular Immunology - Volume 324, February 2018, Pages 42-49
نویسندگان
Ulises De la Cruz-Mosso, Trinidad GarcÃa-Iglesias, Richard Bucala, Iris Estrada-GarcÃa, Laura González-López, Sergio Cerpa-Cruz, Isela Parra-Rojas, Jorge Iván Gámez-Nava, Edsaúl Emilio Pérez-Guerrero, José Francisco Muñoz-Valle,