Importance of NKG2D-NKG2D ligands interaction for cytolytic activity of natural killer cell

► We investigate the necessity of cancer cell markers on NK cell cytotoxicity. ► We found that more susceptible cancer cell lines express more NKG2D ligands. ► The killing activity of NK cell was abolished by addition of a NKG2D antibody. ► NK cell cytolytic activity was increased by upregulating of NKG2D ligands expression.