کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8470609 | 1550005 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Penicillium oxalicum PoFlbC regulates fungal asexual development and is important for cellulase gene expression
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کلمات کلیدی
Penicillium oxalicum4-nitrophenyl-β-d-glucopyranosidepNPGDNSCMC-NaPAGERNA-seqCCR3,5-dinitrosalicylic acid - اسید 3،5-دینیتروسالتیسیلیکpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدRNA sequencing - ترتیب RNACarbon catabolite repression - سرکوب کاتابولیت کربنCellulase - سلولازTranscription factor - عامل رونویسیConidiation - مسکنwild type - نوع وحشیHemicellulase - همی سلولزsodium carboxymethyl cellulose - کربوکسی متیل سلولز سدیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Filamentous fungi can initiate vegetative growth on complex plant polysaccharides in nature through secreting a large amount of lignocellulose-degrading enzymes. These fungi develop a large amount of asexual spores to disperse and survive under harsh conditions, such as carbon and nitrogen depletion. Numerous studies report the presence of a cross-talk between asexual development and extracellular enzyme production, especially at the regulation level. This study identified and characterized a C2H2-type transcription factor called PoFlbC, which is an Aspergillus FlbC ortholog, in cellulolytic fungus Penicillium oxalicum. Results showed that the native level of PoFlbC was crucial for the normal growth and asexual development of P. oxalicum. Importantly, deletion of the PoflbC gene substantially reduced cellulase and hemicellulase productions. Comparative transcriptome analysis by RNA sequencing revealed a global downregulation of genes encoding cellulases, hemicellulases, and other proteins with functions in lignocellulose degradation. A similar defect was also observed in the OEPoflbC strain, suggesting that the production of cellulolytic enzymes was maintained by native expression of the PoflbC. In this study, an essential activator for both fungal asexual development and cellulase production was established in P. oxalicum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 86, January 2016, Pages 91-102
Journal: Fungal Genetics and Biology - Volume 86, January 2016, Pages 91-102
نویسندگان
Guangshan Yao, Zhonghai Li, Ruimei Wu, Yuqi Qin, Guodong Liu, Yinbo Qu,