کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8470623 | 1550009 | 2015 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of cytochrome b5 reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence
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کلمات کلیدی
CyPCYP51FPKMLCBRNA-seqSeptoria tritici blotchSeptoria triticiMycosphaerella graminicolaCBRDPIGC–MS - GC-MSCPR - احیای قلبی ریویFatty acids - اسیدهای چربRNA sequencing - ترتیب RNAdays post inoculation - روز بعد از تلقیحcytochrome b5 - سیتوکروم B5Cytochrome b5 reductase - سیتوکروم B5 ردوکتازcytochrome P450 reductase - سیتوکروم P450 ردوکتازCytochrome P450 - سیتوکروم پی۴۵۰gas chromatography–mass spectrometry - طیف سنجی جرم کروماتوگرافی گازDimorphic fungi - قارچهای دیمورفیکFatty acid methyl ester - متیل استر اسید چربFAME یا fatty acid methyl esters - متیل استرهای اسید چربwild-type - نوع وحشیSTB - و غیره.High pressure liquid chromatography - کروماتوگرافی مایع با فشار بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Analysis of cytochrome b5 reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence Analysis of cytochrome b5 reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence](/preview/png/8470623.png)
چکیده انگلیسی
Septoria tritici blotch (STB) caused by the Ascomycete fungus Zymoseptoria tritici is one of the most economically damaging diseases of wheat worldwide. Z. tritici is currently a major target for agricultural fungicides, especially in temperate regions where it is most prevalent. Many fungicides target electron transfer enzymes because these are often important for cell function. Therefore characterisation of genes encoding such enzymes may be important for the development of novel disease intervention strategies. Microsomal cytochrome b5 reductases (CBRs) are an important family of electron transfer proteins which in eukaryotes are involved in the biosynthesis of fatty acids and complex lipids including sphingolipids and sterols. Unlike the model yeast Saccharomyces cerevisiae which possesses only one microsomal CBR, the fully sequenced genome of Z. tritici bears three possible microsomal CBRs. RNA sequencing analysis revealed that ZtCBR1 is the most highly expressed of these genes under all in vitro and in planta conditions tested, therefore ÎZtCBR1 mutant strains were generated through targeted gene disruption. These strains exhibited delayed disease symptoms on wheat leaves and severely limited asexual sporulation. ÎZtCBR1 strains also exhibited aberrant spore morphology and hyphal growth in vitro. These defects coincided with alterations in fatty acid, sphingolipid and sterol biosynthesis observed through GC-MS and HPLC analyses. Data is presented which suggests that Z. tritici may use ZtCBR1 as an additional electron donor for key steps in ergosterol biosynthesis, one of which is targeted by azole fungicides. Our study reports the first functional characterisation of CBR gene family members in a plant pathogenic filamentous fungus. This also represents the first direct observation of CBR functional ablation impacting upon fungal sterol biosynthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 82, September 2015, Pages 69-84
Journal: Fungal Genetics and Biology - Volume 82, September 2015, Pages 69-84
نویسندگان
Mark C. Derbyshire, Louise Michaelson, Josie Parker, Steven Kelly, Urvashi Thacker, Stephen J. Powers, Andy Bailey, Kim Hammond-Kosack, Mikael Courbot, Jason Rudd,