کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8470698 | 1550010 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Candida albicans autophagy, no longer a bystander: Its role in tolerance to ER stress-related antifungal drugs
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کلمات کلیدی
ERADAutophagosomeUPRATGCVTAutophagy - اتوفاژیER stress - استرس استTem - این استER-Associated Degradation - تخریب ER-AssociatedAntifungal drug - داروهای ضد قارچCer - سرendoplasmic reticulum - شبکه آندوپلاسمی ERA - عصرPlasma membrane - غشای پلاسماTransmission electron microscopy - میکروسکوپ الکترونی عبوریUnfolded protein response - پاسخ پروتئین آشکارCandida albicans - کاندیدا آلبیکنس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Autophagy is a degradation process involved in pathogenicity of many pathogenic fungi. However, its roles in Candida albicans, the leading fungal pathogen in human beings, remain to be detailed. Most recently, we found that endoplasmic reticulum (ER) stress-inducing conditions led to transcriptional up-regulation of C. albicans autophagy-related (ATG) genes, implying a possible link between autophagy and ER stress response in this pathogen. Using a series of C. albicans ATG mutants and autophagy reporting systems, we found that both treatment of ER stress-related drugs and loss of the ER calcium pump Spf1 promoted autophagic flux of Atg8 and Lap41 (a homologue of Saccharomyces cerevisiae Ape1), indicating that these conditions induce autophagy. Moreover, deletion of ATG genes in the spf1Î/Î mutant rendered cells hypersensitive to these drugs and caused activation of UPR, revealing a role of autophagy in alleviating ER stress. In addition, only treatment of tunicamycin and loss of Spf1 in combination increased autophagic flux of the ER component Sec63, suggesting that most of the ER stress-related conditions cause non-selective autophagy rather than selective ER phagy. This study uncovers the important role of C. albicans autophagy in ER stress response and tolerance to antifungal drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 81, August 2015, Pages 238-249
Journal: Fungal Genetics and Biology - Volume 81, August 2015, Pages 238-249
نویسندگان
Qilin Yu, Chang Jia, Yijie Dong, Bing Zhang, Chenpeng Xiao, Yulu Chen, Yuzhou Wang, Xiaoling Li, Lei Wang, Biao Zhang, Mingchun Li,