کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8471036 | 1550046 | 2018 | 39 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization, tissue distribution, localization and mRNA expression of the bucky ball gene in the Dabry's sturgeon (Acipenser dabryanus) during oogenesis
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کلمات کلیدی
DAPIMS-222PGCsBalbiani bodyDPH4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولmitochondrial cloud - ابرهای میتوکندریExpression - اصطلاحLocalization - بومی سازیrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدbase pairs - جفت پایهdays post-hatching - روز بعد از اخراجprimordial germ cells - سلول های بنیادی اولیهfluorescent in situ hybridization - فلورسنت در محل hybridizationFish - ماهیRace - مسابقهUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهSMART - هوشمندانهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In many organisms, germ cells are specified during embryogenesis by the inheritance of maternally deposited RNAs and proteins termed germ plasm. In vertebrates, the bucky ball (buc) gene plays an essential role in the germ plasm aggregation. In this study, the full-length cDNA of buc homologue in Dabry's sturgeon, Adbuc, was isolated and characterized. Multiple sequence alignments showed that the BUVE domain of Buc was highly conserved in vertebrates, despite exhibiting low identities with each other across the whole protein. By quantitative real-time PCR analysis, we found that Adbuc RNAs were only detected in the gonad with a high level in the ovary and a very low level in the testis. During embryogenesis, these RNAs were highly expressed from the unfertilized eggs to blastula, declined dramatically from the gastrula stage, and hardly found after the neurula stage. Moreover, with the development of ovary, the expression level of Adbuc was increasing. By in situ hybridization, the signal of Adbuc was not found in the oogonia, increased slightly in the stage I oocytes, and extremely strong in the stage II oocytes, suggesting that the signal became much stronger with increasing size of oocytes. Additionally, Adbuc co-localized with the mitochondrial cloud. Thus, we conclude that Dabry's sturgeon buc gene might also function in germplasm formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 28, June 2018, Pages 62-71
Journal: Gene Expression Patterns - Volume 28, June 2018, Pages 62-71
نویسندگان
Huan Ye, Huamei Yue, Xiaoge Yang, Chuangju Li, Qiwei Wei,