کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8473853 | 1550413 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New insights on pyrimidine signalling within the arterial vasculature - Different roles for P2Y2 and P2Y6 receptors in large and small coronary arteries of the mouse
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کلمات کلیدی
adenosine-5′-O-(3-thiotriphosphate)UDPEctonucleoside triphosphate diphosphohydrolasePGF2αEmaxRmaxUTPATPγSMABPET-1P2Y6EC50P2Y2PSSuridine diphosphate - uridine دی فسفاتAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPendothelin-1 - اندوتلین-1Uridine triphosphate - اوریدین تری فسفاتphysiological saline solution - راه حل فیزیولوژیکی شورmean arterial blood pressure - فشار خون شریانیLAD - لادوKnock-out mice - موش نابود شدهknock-out - ناک اوتwild type - نوع وحشیNitric oxide - نیتریک اکسیدProstaglandin F2α - پروستاگلاندین F2αcoronary circulation - گردش خون کرونریPurinergic receptors - گیرنده های Purinergic
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Extracellular pyrimidines activate P2Y receptors on both smooth muscle cells and endothelial cells, leading to vasoconstriction and relaxation respectively. The aim of this study was to utilize P2Y knock-out (KO) mice to determine which P2Y receptor subtype are responsible for the contraction and relaxation in the coronary circulation and to establish whether P2Y receptors have different functions along the mouse coronary vascular tree. We tested stable pyrimidine analogues on isolated coronary arteries from P2Y2 and P2Y6 receptor KO mice in a myograph setup. In larger diameter segments of the left descending coronary artery (LAD) (lumen diameter ~ 150 μm) P2Y6 is the predominant contractile receptor for both UTP (uridine triphosphate) and UDP (uridine diphosphate) induced contraction. In contrast, P2Y2 receptors mediate endothelial-dependent relaxation. However, in smaller diameter LAD segments (lumen diameter ~ 50 μm), the situation is opposite, with P2Y2 being the contractile receptor and P2Y6 functioning as a relaxant receptor along with P2Y2. Immunohistochemistry was used to confirm smooth muscle and endothelial localization of the receptors. In vivo measurements of blood pressure in WT mice revealed a biphasic response to the stable analogue UDPβS. Based on the changes in P2Y receptor functionality along the mouse coronary arterial vasculature, we propose that UTP can act as a vasodilator downstream of its release, after being degraded to UDP, without affecting the contractile pyrimidine receptors. We also propose a model, showing physiological relevance for the changes in purinergic receptor functionality along the mouse coronary vascular tree.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 93, April 2016, Pages 1-11
Journal: Journal of Molecular and Cellular Cardiology - Volume 93, April 2016, Pages 1-11
نویسندگان
Kristian Agmund Haanes, Stine Spray, Susanne Syberg, Niklas Rye Jørgensen, Bernard Robaye, Jean-Marie Boeynaems, Lars Edvinsson,