کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8473922 | 1550414 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene signatures of postoperative atrial fibrillation in atrial tissue after coronary artery bypass grafting surgery in patients receiving β-blockers
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کلمات کلیدی
EGFRPitx2RAAGRK5eQTLGSEAGSHCPBFDRpaired-like homeodomain 2TGF-β1NF-κBVesicularnESOxidant stress - استرس اکسیدانEnrichment Score - امتیاز غنی سازیcardiopulmonary bypass - بایپس قلب و ریهβ-blocker - بتا بلوکر کنندهExpression quantitative trait loci - بیان صفات صفات کمیtransforming growth factor beta 1 - تبدیل فاکتور رشد بتا 1Gene Set Enrichment Analysis - تجزیه و تحلیل غنی سازی مجموعه ژنیcoronary artery bypass grafting - تجویز بایپس عروق کرونرCABG - جراحی کنارگذر سرخرگ تاجیKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوright atrial appendage - راست تقریبا دهلیزیnuclear factor-kappa beta - فاکتور هسته ای - کاپا بتاAtrial fibrillation - فیبریلاسیون دهلیزیRedox modulation - مدولاسیون Redoxfalse discovery rate - میزان کشف کاذبnormalized enrichment score - نمره غنی سازی نرمال شدهSingle nucleotide polymorphisms - پلیمورفیسم تک نوکلئوتیدیPegasus - پگاسوسCoronary artery bypass graft - پیوند عروق کرونرSNP - چندریختی تک-نوکلئوتیدquality control - کنترل کیفیتGlutathione - گلوتاتیونEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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![عکس صفحه اول مقاله: Gene signatures of postoperative atrial fibrillation in atrial tissue after coronary artery bypass grafting surgery in patients receiving β-blockers Gene signatures of postoperative atrial fibrillation in atrial tissue after coronary artery bypass grafting surgery in patients receiving β-blockers](/preview/png/8473922.png)
چکیده انگلیسی
Atrial tissue gene expression profiling may help to determine how differentially expressed genes in the human atrium before cardiopulmonary bypass (CPB) are related to subsequent biologic pathway activation patterns, and whether specific expression profiles are associated with an increased risk for postoperative atrial fibrillation (AF) or altered response to β-blocker (BB) therapy after coronary artery bypass grafting (CABG) surgery. Right atrial appendage (RAA) samples were collected from 45 patients who were receiving perioperative BB treatment, and underwent CABG surgery. The isolated RNA samples were used for microarray gene expression analysis, to identify probes that were expressed differently in patients with and without postoperative AF. Gene expression analysis was performed to identify probes that were expressed differently in patients with and without postoperative AF. Gene set enrichment analysis (GSEA) was performed to determine how sets of genes might be systematically altered in patients with postoperative AF. Of the 45 patients studied, genomic DNA from 42 patients was used for target sequencing of 66 candidate genes potentially associated with AF, and 2,144 single-nucleotide polymorphisms (SNPs) were identified. We then performed expression quantitative trait loci (eQTL) analysis to determine the correlation between SNPs identified in the genotyped patients, and RAA expression. Probes that met a false discovery rate < 0.25 were selected for eQTL analysis. Of the 17,678 gene expression probes analyzed, 2 probes met our prespecified significance threshold of false discovery rate < 0.25. The most significant probe corresponded to vesicular overexpressed in cancer - prosurvival protein 1 gene (VOPP1; 1.83 fold change; P = 3.47 Ã 10â 7), and was up-regulated in patients with postoperative AF, whereas the second most significant probe, which corresponded to the LOC389286 gene (0.49 fold change; P = 1.54 Ã 10â 5), was down-regulated in patients with postoperative AF. GSEA highlighted the role of VOPP1 in pathways with biologic relevance to myocardial homeostasis, and oxidative stress and redox modulation. Candidate gene eQTL showed a trans-acting association between variants of G protein-coupled receptor kinase 5 gene, previously linked to altered BB response, and high expression of VOPP1. In patients undergoing CABG surgery, RAA gene expression profiling, and pathway and eQTL analysis suggested that VOPP1 plays a novel etiological role in postoperative AF despite perioperative BB therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 92, March 2016, Pages 109-115
Journal: Journal of Molecular and Cellular Cardiology - Volume 92, March 2016, Pages 109-115
نویسندگان
Miklos D. Kertai, Wenjing Qi, Yi-Ju Li, Frederick W. Lombard, Yutao Liu, Michael P. Smith, Mark Stafford-Smith, Mark F. Newman, Carmelo A. Milano, Joseph P. Mathew, Mihai V. Podgoreanu,