کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8474394 | 1550425 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acute reversal of phospholamban inhibition facilitates the rhythmic whole-cell propagating calcium waves in isolated ventricular myocytes
ترجمه فارسی عنوان
واکنش حاد مهار فسفولامبن موجب تشدید امواج کلسیم در کل سلول های ریتمیک در میوسیت های جدا شده بطنی می شود
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
Here, we used the Fab fragment of 2D12, a monoclonal anti-PLB antibody, to test how acute reversal of PLB inhibition affects the spontaneous SR Ca2Â + release in normal VMs. Ca2Â + sparks and spontaneous Ca2Â + waves (SCWs) were recorded in the line-scan mode of confocal microscopy using the Ca2Â + fluorescent dye Fluo-4 in isolated permeabilized mouse VMs. Fab, which reverses PLB inhibition, significantly increased the frequency, amplitude, and spatial/temporal spread of Ca2Â + sparks in VMs exposed to 50Â nM free [Ca2Â +]. At physiological diastolic free [Ca2Â +] (100-200Â nM), Fab facilitated the formation of whole-cell propagating SCWs. At higher free [Ca2Â +], Fab increased the frequency and velocity, but decreased the decay time of the SCWs. cAMP had little additional effect on the frequency or morphology of Ca2Â + sparks or SCWs after Fab addition. These findings were complemented by computer simulations. In conclusion, acute reversal of PLB inhibition alone significantly increased the spontaneous SR Ca2Â + release, leading to the facilitation and organization of whole-cell propagating SCWs in normal VMs. PLB thus plays a key role in subcellular Ca2Â + dynamics and rhythmic activity of VMs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 80, March 2015, Pages 126-135
Journal: Journal of Molecular and Cellular Cardiology - Volume 80, March 2015, Pages 126-135
نویسندگان
Yi-Hsin Chan, Wei-Chung Tsai, Zhen Song, Christopher Y. Ko, Zhilin Qu, James N. Weiss, Shien-Fong Lin, Peng-Sheng Chen, Larry R. Jones, Zhenhui Chen,