High-mobility group box 1 (HMGB1) impaired cardiac excitation-contraction coupling by enhancing the sarcoplasmic reticulum (SR) Ca2Â + leak through TLR4-ROS signaling in cardiomyocytes

High-mobility group box 1 (HMGB1) is a proinflammatory mediator playing an important role in the pathogenesis of cardiac dysfunction in many diseases. In this study, we explored the effects of HMGB1 on Ca2 + handling and cellular contractility in cardiomyocytes to seek for the mechanisms underlying HMGB1-induced cardiac dysfunction. Our results show that HMGB1 increased the frequency of Ca2 + sparks, reduced the sarcoplasmic reticulum (SR) Ca2 + content, and decreased the amplitude of systolic Ca2 + transient and myocyte contractility in dose-dependent manners in adult rat ventricular myocytes. Inhibiting high-frequent Ca2 + sparks with tetracaine largely inhibited the alterations of SR load and Ca2 + transient. Blocking Toll-like receptor 4 (TLR4) with TAK-242 or knockdown of TLR4 by RNA interference remarkably inhibited HMGB1 induced high-frequent Ca2 + sparks and restored the SR Ca2 + content. Concomitantly, the amplitude of systolic Ca2 + transient and myocyte contractility had significantly increased. Furthermore, HMGB1 increased the level of intracellular reactive oxygen species (ROS) and consequently enhanced oxidative stress and CaMKII-activated phosphorylation (pSer2814) in ryanodine receptor 2 (RyR2). TAK-242 pretreatment significantly decreased intracellular ROS levels and oxidative stress and hyperphosphorylation in RyR2, similar to the effects of antioxidant MnTBAP. Consistently, MnTBAP normalized HMGB1-impaired Ca2 + handling and myocyte contractility. Taken together, our findings suggest that HMGB1 enhances Ca2 + spark-mediated SR Ca2 + leak through TLR4-ROS signaling pathway, which causes partial depletion of SR Ca2 + content and hence decreases systolic Ca2 + transient and myocyte contractility. Prevention of SR Ca2 + leak may be an effective therapeutic strategy for the treatment of cardiac dysfunction related to HMGB1 overproduction.