کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8479277 | 1551309 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antioxidant action of 7,8-dihydroxyflavone protects PC12 cells against 6-hydroxydopamine-induced cytotoxicity
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کلمات کلیدی
MDAECLATCCMPTP6-HydroxydopamineHRPFCS7,8-DHFDMEM6-OHDA1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine - 1-methyl-4-phenyl-1،2،3،6-tetrahydropyridine7,8-Dihydroxyflavone - 7،8-دی هیدروکسی فلاونDMSO - DMSOMTT - MTTHydrogen peroxide - آب اکسیژنهenhanced chemiluminescence - بهبود شیمیایی لومنAlzheimer’s disease - بیماری آلزایمرThiazolyl blue tetrazolium bromide - تترازولیم برومید تیزولیل آبیANOVA - تحلیل واریانس Analysis of varianceone-way analysis of variance - تحلیل واریانس یک راههDopamine - دوپامینDimethyl sulfoxide - دیمتیل سولفواکسیدfetal calf serum - سرم گوساله جنینmalondialdehyde - مالون دی آلدهیدAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoH2O2 - هیدروژن پراکسیدHorseradish peroxidase - پراکسیداز هوررادیش
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Antioxidant action of 7,8-dihydroxyflavone protects PC12 cells against 6-hydroxydopamine-induced cytotoxicity Antioxidant action of 7,8-dihydroxyflavone protects PC12 cells against 6-hydroxydopamine-induced cytotoxicity](/preview/png/8479277.png)
چکیده انگلیسی
Oxidative stress-induced neuronal death plays a pivotal role in pathogenesis of neurodegenerative disorders. Recently, 7,8-dihydroxyflavone (7,8-DHF) has been shown to exert neuroprotective effects by acting as a selective tyrosine kinase receptor B (TrkB) agonist. In addition, the antioxidant action of 7,8-DHF may protect neuronal cells against oxidative injury. In the present study, we used PC12 cells, a cell line generally thought to lack TrkB, to investigate the effect of 7,8-DHF on 6-hydroxydopamine (6-OHDA)-induced cytotoxicity and the underlying mechanism. We found that 7,8-DHF effectively prevented cell death, apoptosis and mitochondrial dysfunction induced by 6-OHDA. In a cell free system, 7,8-DHF did not slow down extracellular auto-oxidation of 6-OHDA which may generate H2O2. However, We found that 7,8-DHF dramatically reduced cellular malondialdehyde content and phospho-histone H2A.X protein level. 7,8-DHF also elevated total superoxide dismutase activity in 6-OHDA-treated cells. These results indicate that 7,8-DHF might protect PC12 cells against 6-OHDA-induced cytotoxicity through its powerful antioxidant activity. By acting as a potent TrkB agonist and an antioxidant together with its easiness to pass across blood-brain barrier, 7,8-DHF may be developed into a promising candidate in treatment of neurodegenerative diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 64, January 2014, Pages 18-23
Journal: Neurochemistry International - Volume 64, January 2014, Pages 18-23
نویسندگان
Xiaohua Han, Shaolei Zhu, Bingxiang Wang, Lei Chen, Ran Li, Weicheng Yao, Zhiqiang Qu,