کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8491223 | 1552369 | 2016 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design, implementation and interpretation of in vitro batch culture experiments to assess enteric methane mitigation in ruminants-a review
ترجمه فارسی عنوان
طراحی، پیاده سازی و تفسیر تجربیات آزمایشگاهی کشت نمونه های درون سلولی جهت ارزیابی اثرات کاهش متان در مجاری گوسفند - بررسی
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کلمات کلیدی
DMIVFAMicrobial inoculumNDFFeed evaluation - ارزیابی خوراکVolatile fatty acids - اسیدهای چرب فرارMitigation - تسکین دهندهIn vitro gas production - تولید گاز درون آزمایشگاهیRumen - شکمبه، سیرابیneutral detergent fibre - فیبر مواد شوینده خنثیorganic matter - ماده آلیdry matter - ماده خشکMethane - متان dry matter intake - مصرف ماده خشک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
In vitro fermentation techniques (IVFT) have been widely used to evaluate the nutritive value of feeds for ruminants and in the last decade to assess the effect of different nutritional strategies on methane (CH4) production. However, many technical factors may influence the results obtained. The present review has been prepared by the 'Global Network' FACCE-JPI international research consortium to provide a critical evaluation of the main factors that need to be considered when designing, conducting and interpreting IVFT experiments that investigate nutritional strategies to mitigate CH4 emission from ruminants. Given the increasing and wide-scale use of IVFT, there is a need to critically review reports in the literature and establish what criteria are essential to the establishment and implementation of in vitro techniques. Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements. Based on an evaluation of experimental evidence, a set of technical recommendations are presented to harmonize IVFT for feed evaluation, assessment of rumen function and CH4 production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Feed Science and Technology - Volume 216, June 2016, Pages 1-18
Journal: Animal Feed Science and Technology - Volume 216, June 2016, Pages 1-18
نویسندگان
Yáñez-Ruiz D.R., Bannink A., Dijkstra J., Kebreab E., Morgavi D.P., O'Kiely P., Reynolds C.K., Schwarm A., Shingfield K.J., Yu Z., Hristov A.N.,