کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8492920 | 1552792 | 2018 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of muscle-specific creatine kinase expression as physiological indicator for Atlantic salmon (Salmo salar L) skeletal muscle damage
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
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چکیده انگلیسی
Plasma creatine kinase (CK) release is a marker of damage in fish; their expression can indicate drug/contaminant exposure. Three CK isoforms have been described in fish: muscle CK (M-CK), brain CK (B-CK) and mitochondrial sarcomeric, and the release of any of them could be detected in the serum. Nevertheless, the expression and properties of M-CK in Atlantic salmon are still unknown. In this study, we evaluated differential M-CK expression in Atlantic salmon (Salmo salar L) skeletal muscle and serum at 0, 5, 10, and 15â¯days post-infection (dpi) with the infectious salmon anaemia virus, a primary aquaculture pathogen in Chile. The M-CK isoform was assessed using a Western blot-validated antibody. Results for qPCR (+10.8â¯AU) and ELISA (1.24, pâ¯<â¯0.05) showed increased skeletal muscle M-CK at 10 dpi (râ¯=â¯0.9817, pâ¯<â¯0.05). Serum M-CK significantly increased at 5 and 10 dpi (râ¯=â¯1.26 and 1.17, pâ¯<â¯0.05). In situ M-CK detection in the myofibrils showed co-localization with the pro-inflammatory cytokine IL-1β, particularly at 10 dpi. In summary, serum M-CK detection apparently serves as a non-invasive, functional indicator for Atlantic salmon skeletal muscle damage, which could indicate disease or environmental stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 496, 1 November 2018, Pages 66-72
Journal: Aquaculture - Volume 496, 1 November 2018, Pages 66-72
نویسندگان
Verónica Rojas, Byron Morales-Lange, Rubén Avendaño-Herrera, MatÃas Poblete-Morales, Diana Tapia-Cammas, Fanny Guzmán, Sergio H. Marshall, Luis Mercado,