کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8493620 | 1552805 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)](/preview/png/8493620.png)
چکیده انگلیسی
Study in semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the semen collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of semen collection via digital manipulation of the penis. Four matured male crocodiles kept in the same enclosure were physically restrained for semen collection. The collection was done in January 2015 during crocodiles mating season. Mean (± SEM) seminal volume, mass movement, pH, sperm concentration, general motility, forward movement, rotating and vibrating movements measured were 0.55 ± 0.12 ml, 2.75 ± 0.48, 7.13 ± 0.24, 396.88 ± 43.41 Ã 106 sperm/ml, 45 ± 17.56%, 37.13 ± 5.12%, 9.56 ± 5.38% and 53.13 ± 1.92% respectively. The most important sperm abnormalities that requires further investigation is the cytoplasmic droplets which was 28.25 ± 2.29%. Sperm motility trend was best preserved in extender made up of 0.01 M PBS with 5% egg yolk. Sperm stored 4 °C in this extender had survived longer than the other two extender which was 11 days. There is a unique trend in the spike of spermatozoa motility post 48 h in chilled condition however the cause of such pattern is still unknown. Semen collection by digital manipulation of the penis was adequate in obtaining semen and the semen characteristics of the estuarine crocodile are described.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 483, 20 January 2018, Pages 169-172
Journal: Aquaculture - Volume 483, 20 January 2018, Pages 169-172
نویسندگان
Wan-Nor Fitri, Haron Wahid, Putra Tengku Rinalfi, Yusoff Rosnina, Dana Raj, Yawah Donny, Latip Qayyum, Ab Aziz Abdul Malek,