کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8494853 1552852 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Water hardness influences Flavobacterium columnare pathogenesis in channel catfish
ترجمه فارسی عنوان
سختی آب بر پاتوژنز فلووباکتریوم ستون در کانال گربه تاثیر می گذارد
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
چکیده انگلیسی
Studies were conducted to determine aspects of water chemistry responsible for large differences in pathogenesis and mortality rates in experimental challenges of channel catfish Ictalurus punctatus with Flavobacterium columnare; challenges were conducted in water supplying either the Stuttgart National Aquaculture Research Center (SNARC) in Stuttgart, Arkansas, or the Warmwater Aquaculture Research Unit (WARU) in Stoneville, Mississippi. Waters differed in two major characteristics: compared to SNARC water, WARU water contained appreciable dissolved organic matter (DOM) and very low concentrations of dissolved calcium and magnesium. In Experiment 1, fish were exposed to a bacterial challenge of F. columnare in aquaria with either SNARC or WARU water. Fish mortality at 4 d was 100% for fish in SNARC water, while no fish died in WARU water. Bacterial adhesion to gill surfaces also differed dramatically in the two waters and was approximately 1900-times higher for fish challenged in SNARC water (more than 800,000 bacterial CFUs/ng extracted DNA) than for fish challenged in WARU water (fewer than 450 CFUs/ng). Experiment 2 was conducted with a lower bacterial challenge to determine which factor, DOM or divalent cation concentration, caused the difference in mortality seen in Experiment 1. Complete removal of DOM from WARU water by carbon filtration did not affect bacterial adhesion to gills nor resulted in greater mortality in challenged fish. Consequently, DOM did not contribute to the differences in mortality seen in Experiment 1. However, removal of most calcium and magnesium from SNARC water by ion-exchange filtration resulted in decreased bacterial adhesion to gill and decreased mortality compared to un-altered SNARC water. It appears that the concentration of divalent cations (hardness) affects the pathogenesis of columnaris disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 435, 1 January 2015, Pages 252-256
نویسندگان
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