کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8495187 | 1552859 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Early individual electronic identification of sea bass using RFID microtags: A first example of early phenotyping of sex-related growth
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
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چکیده انگلیسی
Although individual electronic tagging using PIT (Passive Integrated Transponder) tags is well established, it is mainly used for fish >Â 60Â mm in length. Since electronic tagging is an ideal identification method, we used RFID (Radio Frequency Identification) microtags (6Â mm in length, 1Â mm in diameter, 10Â mg in mass) to characterize individual fish from the early stages of their development and throughout their lifecycles. We used sea bass, Dicentrarchus labrax (105Â day-old fish weighing between 100 and 1100Â mg), and studied the effects of intra-coelomic tagging in half the population using different endpoints including survival and tag reading, growth over 6Â months and swimming responses. Dead fish were counted daily, biometric data were collected at 21 to 28Â days intervals and fish were sexed at the end of the experiment. Behavioral swimming responses following a sudden dark challenge were evaluated after the first three biometric measurements (immediately after tagging, and then 21 and 42Â days later). After 6Â months, mean survival was 69%, the smallest surviving fish weighed 197Â mg at tagging, and success in tag reading was 79% for the size class 300-400Â mg after 63Â days. No negative effects were observed on growth and most deaths occurred within 15Â days after tagging with a first peak after 5Â days but that did not particularly affect the smallest individuals. Differences in swimming responses were detected between tagged fish and untagged controls immediately after the surgical procedure with tagged fish swimming more than controls for 45Â min. However, after 21Â days, the tagged fish swam less than the untagged controls after being placed in the observation tank for 1Â h and 45Â min. At 42Â days post-tagging, no further differences were observed. Overall, results suggested that the tagging method was suitable for fish as small as 400Â mg and 36Â mm in total length. This type of early tagging method is invaluable for the longitudinal monitoring of individual biological traits (e.g., growth) or for repeated assays with the same individual at distinct time points (e.g., behavior studies). We used it for a first evaluation of early growth differentiation between sexes and demonstrated a 31% mass gain difference in females that was visible as soon as the fish reached 105Â days old. This confirms the potential of the method for the selection of early-expressed character traits which could lead to rearing cost savings for the aquaculture research field.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volumes 426â427, 20 April 2014, Pages 165-171
Journal: Aquaculture - Volumes 426â427, 20 April 2014, Pages 165-171
نویسندگان
Sébastien Ferrari, Béatrice Chatain, Xavier Cousin, Didier Leguay, Alain Vergnet, Marie-Odile Vidal, Marc Vandeputte, Marie-Laure Bégout,