Separation of Penaeus vannamei haemocyte subpopulations by iodixanol density gradient centrifugation

This methodology allowed the separation and characterization of five morphologically distinct and physiologically active P. vannamei haemocyte subpopulations, from which three were isolated with a very high degree of purity. Therefore, we consider this methodology a valuable alternative for the traditional crustacean haemocyte separation procedure in Percoll.