کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8495996 | 1552914 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Required dose of fish nervous necrosis virus (NNV) for Poly(I:C) immunization of sevenband grouper Epinephelus septemfasciatus
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
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چکیده انگلیسی
It was recently reported that Poly(I:C) immunization of fish confers protection against viral infection. In this process, fish are immunized with a live pathogenic virus followed by administration of Poly(I:C), which induces a transient, non-specific antiviral state. As a result, fish survive the initial immunization with live virus, which would otherwise be lethal. In the present study, we tried to determine the exact dosage of red-spotted grouper nervous necrosis virus (RGNNV), the causative agent of viral nervous necrosis, required for Poly(I:C) immunization of sevenband grouper Epinephelus septemfasciatus. In Poly(I:C) immunization with more than 90% relative percentage survival (RPS) against RGNNV challenge with 104.3 TCID50/fish, approximately 104.0 TCID50/fish or greater of RGNNV was administered by intramuscular (IM) injection, whereas 105.3 TCID50/fish or greater of RGNNV was required for immersion methods. It was concluded that the degree of RGNNV infection must be similar to a fatal dose in order for the fish to become immune to RGNNV. Interestingly, antibody against RGNNV was not detected in the sera of fish immunized by immersion. Thus, Poly(I:C) immunization by immersion may have some interesting effects on the epidermal immune response in fish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 311, Issues 1â4, 3 February 2011, Pages 100-104
Journal: Aquaculture - Volume 311, Issues 1â4, 3 February 2011, Pages 100-104
نویسندگان
Toyohiko Nishizawa, Ikuo Takami, Mamoru Yoshimizu, Myung-Joo Oh,