Neutralizing assay of monoclonal antibodies against white spot syndrome virus (WSSV)

Monoclonal antibodies (Mabs) against white spot syndrome virus (WSSV) were tested for their ability to neutralize WSSV infectivity by immunodot-blot assay and enzyme-linked immunosorbent assay. It was proved that WSSV could bind to haemocyte membranes of Penaeus chinensis, moreover, the result showed that the Mab 2D2 could reduce the binding of WSSV with haemocyte membranes of P. chinensis. The neutralization assay was further conducted in vitro with primary cultured haemocytes of P. chinensis, and the cell culture assay showed that the two monoclonal antibodies, 2D2 and 6B4 could partially neutralize WSSV infection.