کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8497596 | 1553543 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of another primordial CD80/86 molecule in rainbow trout: Insights into the origin and evolution of CD80 and CD86 in vertebrates
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کلمات کلیدی
mAbHKLCD80/86Elongation factor 1AWGDAPCqPCRORFTCrMonoclonal antibody - آنتی بادی مونوکلونالmye - بسیارWhole-genome duplication - تقلید کامل ژنومduplication - تکثیرDouble negative - دو برابر منفی استantigen presenting cell - سلولهای پردازنده آنتیژنtransmembrane - فرابنفشEvolution - فرگشت open reading frame - قاب خواندن بازRainbow trout - قزل آلای رنگین کمانmya - میاMyeloid - میلوئیدmillion years ago - میلیون سال پیشquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیT cell receptor - گیرنده سلول T
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In mammals, the binding of distinct costimulatory ligands CD80 and CD86 to their receptors is essential for optimal T cell activation. Previous studies have shown that only a single CD80/86 gene exists in rainbow trout (termed rtCD80/86A) and other teleost fish, suggesting that CD80 and CD86 arose by gene duplication in the tetrapod branch, after the separation of fish and tetrapods. However, in this study, another CD80/86 gene has been cloned from rainbow trout, termed rtCD80/86B. The sequence identity between trout CD80/86 is significantly higher than that between CD80 and CD86 in tetrapods, indicating that CD80 and CD86 underwent divergent evolution in vertebrates, especially in tetrapods. Gene synteny analyses showed that the CD80 and CD86 genes are closely located in the same chromosome in tetrapods. However, CD80/86 genes are located in two distinct chromosomes in rainbow trout and Atlantic salmon, suggesting that salmonid CD80/86 genes arose by the salmonid-specific whole-genome duplication (WGD) event. Expression analysis showed that rtCD80/86A was more abundant and inducible than rtCD80/86B in various tissues, indicating the important role of rtCD80/86A in trout immunity. Interestingly, we found that head kidney B cells showed higher expression level of rtCD80/86A and rtCD80/86B when compared with the other leukocytes, suggesting a potential role for trout B cells as antigen-presenting cells (APCs).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 89, December 2018, Pages 73-82
Journal: Developmental & Comparative Immunology - Volume 89, December 2018, Pages 73-82
نویسندگان
Xu-Jie Zhang, Xiang-Yang Zhang, Peng Wang, Yong-An Zhang,