کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8497625 | 1553544 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Both ganglioside GM2 and cholesterol in the cell membrane are essential for Bombyx mori cypovirus cell entry
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کلمات کلیدی
PBSjunctional adhesion molecule 1Ganglioside GM2Junctional adhesion molecule AJAM-AArg-Gly-AspT3DGSLSBmCPVJAM-1GalNAcVAPMβCDMEFsNSPNeu5Ac3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-diphenyltetrazolium bromideMTT - MTTSialic acid - سیالیک اسیدInfectivity - عفونتmethyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmouse embryonic fibroblasts - موش فیبروبلاست جنینیCytoplasmic polyhedrosis virus - ویروس polyhedrosis سیتوپلاسمیNon-structural proteins - پروتئین های غیر ساختاریBombyx mori - کرم ابریشمmembrane cholesterol - کلسترول غشاءGlycosphingolipids - گلیسفینگولایپید ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
Bombyx mori cypovirus (BmCPV) enters permissive cells via clathrin-mediated endocytosis pathway. However, the distinct entry mechanism for BmCPV is still ambiguous. The aim of this study is to investigate the role of gangliosides and cholesterol in BmCPV cell entry. The number of BmCPV virions attached to the cell surface and the expression level of BmCPV vp1 gene was significantly decreased by digestion of terminal sialic acids in gangliosides with neuraminidase (NA). Preincubation of different concentration of ganglioside GM1, GM2 or GM3 with BmCPV prior to infection, the reduction of BmCPV infectivity was found by GM2-treated in a dose-depend manner. BmCPV virions were found to colocalize with GM2 in the cell surface. The infectivity of BmCPV was reduced by anti-GM2 antibody treatment cells. Moreover, BmCPV infection was impaired by depletion of membrane cholesterol with MβCD, but the inhibitory effect of MβCD was restored by supplementing with cholesterol. The number of viral particles attached on the BmN cells was significantly decreased by pretreated with MβCD, and BmCPV infection was inhibited by silencing the expression of 3-hydroxy-3-methylglutaryl-CoA reductase gene (Hmg-r) in cholesterol biosynthesis pathway. These results indicate that ganglioside GM2 and cholesterol in membrane lipid rafts are essential for BmCPV attachment to cell surface for its cell entry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 88, November 2018, Pages 161-168
Journal: Developmental & Comparative Immunology - Volume 88, November 2018, Pages 161-168
نویسندگان
Liyuan Zhu, Xiaolong Hu, Dhiraj Kumar, Fei Chen, Yongjie Feng, Min Zhu, Zi Liang, Lixu Huang, Lei Yu, Jian Xu, Renyu Xue, Guangli Cao, Chengliang Gong,