کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8498019 | 1553571 | 2016 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9 Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9](/preview/png/8498019.png)
چکیده انگلیسی
Recent advances in gene editing techniques have not been exploited in farmed fishes. We established a gene targeting technique, using the CRISPR/Cas9 system in Labeo rohita, a farmed carp (known as rohu). We demonstrated that donor DNA was integrated via homologous recombination (HR) at the site of targeted double-stranded nicks created by CRISPR/Cas9 nuclease. This resulted in the successful disruption of rohu Toll-like receptor 22 (TLR22) gene, involved in innate immunity and exclusively present in teleost fishes and amphibians. The null mutant, thus, generated lacked TLR22 mRNA expression. Altogether, this is the first evidence that the CRISPR/Cas9 system is a highly efficient tool for targeted gene disruption via HR in teleosts for generating model large-bodied farmed fishes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 61, August 2016, Pages 242-247
Journal: Developmental & Comparative Immunology - Volume 61, August 2016, Pages 242-247
نویسندگان
Vemulawada Chakrapani, Swagat Kumar Patra, Rudra Prasanna Panda, Kiran Dashrath Rasal, Pallipuram Jayasankar, Hirak Kumar Barman,